A novel efficient beta-glucanase from a paddy soil microbial metagenome   with versatile activities

doi:10.1186/s13068-016-0449-6

	A novel efficient beta-glucanase from a paddy soil microbial metagenome with versatile activities
	Zhou Yu ; Wang Xu ; Wei Wei ; Xu Jimin ; Wang Wei ; Xie Zhongwen ; Zhang Zhengzhu ; Jiang Hongchen ; Wang Qi ; Wei Chaoling
	2016
发表期刊	BIOTECHNOLOGY FOR BIOFUELS
卷号	9 期号:1 页码:36
摘要	Background: Cellulose, an abundant and renewable polysaccharides, constitutes the largest resource for bioconversion of biofuels. Plant polysaccharides hydrolysis is catalyzed by cellulases, which include endoglucanases, exoglucanases, and a-glucosidases. Converting cellulose and hemicellulose to short chains of oligosaccharides by endo-/exoglucanases is the key step for biofuel transformation. Intriguingly, beta-glucanases with transglycosylation activity not only can relieve product inhibition of glucan hydrolysis but also has potential application as biocatalysts for functional materials. Results: Here, a metagenomic fosmid library was constructed from a paddy soil for cellulase screening. One purified clone showing carboxymethylcellulase activity was isolated, and the complete beta-glucanase gene (umcel9y-1) was cloned and overexpressed in Escherichia coli. Phylogenetic analysis indicated that beta-glucanase Umcel9y-1 belonged to the theme C of glycoside hydrolase family 9. Amino acids sequence showed 58.4 % similarity between Umcel9y-1 and its closest characterized reference, cellulase Cel01. Biological characterization showed that Umcel9y-1 was an efficient endoglucanase and also exhibited high activities of exoglucanase and transglycosylation. The transglycosylation products of Umcel9y-1 including sophorose, laminaribiose, and gentiobiose, and transglycosylation was detected under all activated conditions. The order of catalytic efficiency for polysaccharides, cellooligosaccharides, and aryl-beta glycosides was p-nitrophenol-D-cellobioside, barley glucan, cellopentaose, cellotetraose, cellotriose, hydroxyethylcellulose, cellohexose, laminarin, and carboxymethylcellulose, respectively. The barley glucan was the optimal polysaccharides for Umcel9y-1 with K-m and K-cat/K-m values of 13.700 mM and 239.152 s(-1) mM(-1), respectively. Conclusion: Biological characterizations of recombinant Umcel9y-1 showed that the versatile beta-glucanase had efficient endoglucanase activity to barley glucan and also exhibited high activities of exoglucanase and transglycosylation. The optimum conditions of recombinant Umcel9y-1 was pH 6.5-7.0 at 37 degrees C with predominant halotolerance and high-thermal stability. These results indicate that the novel metagenomic-derived beta-glucanase may be a potent candidate for industrial applications.
关键词	Metagenomic library Versatile beta-glucanase Glycoside hydrolase family 9 Transglycosylation Endoglucanase Exoglucanases Endoglucanase,Exoglucanases,Glycoside hydrolase fa
DOI	10.1186/s13068-016-0449-6
语种	英语
ISSN	1754-6834
收录类别	PubMed ; SCI ; EI
EI入藏号	20170103218442
WOS记录号	WOS:000369947900001
WOS类目	Biotechnology & Applied Microbiology; Energy & Fuels
出版者	BIOMED CENTRAL LTD
出版地	236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND
PubMed记录号	26877766
引用统计	被引频次：566[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.cug.edu.cn/handle/2XU834YA/279055
专题	教学院系_地理与信息工程学院
作者单位	1.Anhui Agr Univ, Sch Tea & Food Sci Technol, State Key Lab Tea Biol & Utilizat, Hefei 230036, Peoples R China. 2.Zhejiang Acad Agr Sci, Inst Qual & Standard Agroprod, Hangzhou 310021, Zhejiang, Peoples R China. 3.China Univ Geosci, State Key Lab Biogeol & Environm Geol, Wuhan 430074, Peoples R China. 4.Novus Int Shanghai Inc, Shanghai 200080, Peoples R China.
推荐引用方式 GB/T 7714	Zhou Yu,Wang Xu,Wei Wei,et al. A novel efficient beta-glucanase from a paddy soil microbial metagenome with versatile activities[J]. BIOTECHNOLOGY FOR BIOFUELS,2016,9(1):36.
APA	Zhou Yu.,Wang Xu.,Wei Wei.,Xu Jimin.,Wang Wei.,...&Wei Chaoling.(2016).A novel efficient beta-glucanase from a paddy soil microbial metagenome with versatile activities.BIOTECHNOLOGY FOR BIOFUELS,9(1),36.
MLA	Zhou Yu,et al."A novel efficient beta-glucanase from a paddy soil microbial metagenome with versatile activities".BIOTECHNOLOGY FOR BIOFUELS 9.1(2016):36.